signs-your-colony-is-queenless

http://beekeepinglikeagirl.com/signs-your-colony-is-queenless/

1. Lack of Eggs & Brood

2. A Drop in Population

3. An Increase in Honey & Pollen

4. A Change in Temperament

5. Queen Cells/Cups

6. Laying Workers

How to Test for Queenlessness

Give your bees a frame of open brood from a queenright colony. 

Place a caged queen on the tops of the frames and study the reaction.